Every batch synthesized under our supervision undergoes dual analytical verification: High-Performance Liquid Chromatography (HPLC) for purity and Electrospray Ionization Mass Spectrometry (ESI-MS) for molecular identity confirmation.
We utilize automated solid-phase peptide synthesis (SPPS) with heated microwave technology. This facilitates complete deprotection and coupling, preventing difficult sequence aggregation and yielding optimal raw crude purity before purification.
Crude peptides are purified on large-scale preparative reverse-phase HPLC columns with linear gradient elution (acetonitrile/water with 0.1% TFA). Only target fractions exceeding 99.0% UV peak area are collected.
Independent accredited analytical laboratories (such as Janoshik Analytical and MZ Biolabs) perform blind testing on finished lyophilized vials to verify purity percentages, absence of truncated fragments, and exact mass confirmation.
Unlike vendors who rely on supplier-provided certificates from overseas manufacturers, Alchemia Research pulls randomized sample vials from every finished batch and dispatches them directly to independent domestic and European testing facilities.
The resulting chromatograms and spectrometer readings are published unedited to our public COA Index, linked with matching batch lot numbers printed directly on the product labels and presentation boxes.